Tibbiyot Dunyosi has established 5 blood sampling centres in Tashkent. These centres are located in State hospitals and clinics for the convenience of patients. High quality diagnosis and precision is guaranteed. Tibbiyot Dunyosi  plans to open an additional number of centres In the near future, bringing the total number of centres to 10 – 12.

The feasibility of the project

Tibbiyot Dunyosi Laboratory,  with equipment from leading manufacturers, ensures highly accurate analysis. The equipment is serviced by an * LIS program, assigning the patient a bar code when registering. Total confidentiality of information is assured and the system is able to send analysis results to the place of sampling electronically. Using the barcode LIS program also eliminates the possibility of errors due to human factors. 

Service at such a high level in government hospitals and clinics is not possible due to the lack of required laboratory and diagnostic equipment.

In addition, the sampling centre at a medical facility in the community will save a patient time because of the delivery of the analysis and findings to an external laboratory where his sample was taken. The patient will not have to go to the lab twice, and then back to the reception to his doctor.

At the centres of analysis we only offer those kinds of analyses that the clinics and hospitals where these centres are located can not carry out on their own. This will make it easier to monitor the competitiveness and the quality of the analyses. Our plan is to provide possible assistance to medical facilities in the territories where a centre for biomaterials collection exists.

• Free analysis in the amount of 2% of the volume of our services
• Free laboratory products for laboratories in the amount of 50 million soums
• An annual event for medical institutions using spatulas – a donation of one million spatulas in the amount of 234 million soums
• Free supply of hub cutters and needles and sharps containers to reduce the risk of infecting medical personnel.
• Free vaccination of the medical staff of health facilities exposed to the risk of contracting hepatitis B
• On the spot sampling tests and immediate results for patients critically ill in medical establishments.
• Organize seminars with the participation of well-known scientists to improve the skills of doctors in different areas of the health system

Part of the profits from the activity analysis at the centres of bio-material collection at medical and preventative treatment facilities of Tashkent and Tashkent region, we plan to use to finance the operation of two plants with five incinerators for the destruction of medical waste. The plants are of social importance because their realization will effectively help to fight the spread of infectious and viral diseases.

Centres for sampling tests at health care facilities in Tashkent

For accurate and reliable test results, it is necessary to observe a number of requirements and prepare your body properly. There are some general rules that apply to all kinds of tests and some studies require special preliminary preparation including restrictions on diet and taking medication.

Consultation with a physician or a specialist at Tibbiyot Dunyosi Medical Center on the eve of analysis is required. Tell your doctor about medications you are taking and any procedures that took place recently. This will save you from having to ask a lot of questions about the proper preparation and intended date of the study.

We have prepared a memo with which you can control the preparation of the organism for analysis.

Preparing for the blood test. Basic requirements and recommendations:

• Medication should be stopped at least 14 days before the date of analysis.In studies where the drug concentration of the medicine that you are taking in the blood is to be tested, this is not required.
• If unable to discontinue the medicines for health reasons, tell your doctor or Tibbiyot Dunyosi Medical Center, and inform the doctor at our laboratory.
• Avoid spicy, fried foods, foods high in fat and alcohol for 24 hours prior to testing. Do not smoke after one hour before the analysis.Try to avoid physical strain and psychological stress.It is permitted to drink uncarbonated water only, but if possible, drinking should be avoided. Blood taken in the morning is done strictly on an empty stomach.
• To prevent inaccurate results, do not give blood after physical therapy, instrumental examination, X-ray, ultrasound or massage.

Specific requirements and recommendations in preparation for blood tests: 

Urea

• For 48 hours prior to the test, follow the recommended diet and limit physical activity.Eliminate foods rich in purines from your diet. Limit the intake of liver, kidneys, fish meat, coffee and tea.

 Cholesterol, lipoproteins

• Avoid eating for 14 hours before analysis.If the purpose of the analysis is not for specific lipid-lowering effect of drugs that lower blood lipids, cancel the intake of the drugs two weeks before analysis.

Glucose

• Analysis prohibits the consumption of any beverage other than water.Do not clean your teeth or take drugs.

Glucose tolerance test

• During the three days prior to the test, observe a low-carb diet and eliminate medical treatments and medications. Do not smoke, eat or drink for 14 hours before the test.Research is carried out in the morning on an empty stomach. A Glucose tolerance test should not be performed after surgery, childbirth or stress. Neither should it be done in the presence of inflammatory processes, alcoholic cirrhosis or hepatitis. The test is not recommended during menstruation, diseases of the digestive tract and malabsorption of glucose. During pregnancy, there are special rules for a study of the individual stages of pregnancy such as the rate of previous pregnancies and hereditary factors. Before the test it is necessary to consult a doctor for detailed recommendations which must be strictly implemented.

Hormonal Examination  

• Blood for hormones examination is done in the morning on an empty stomach.For 48 hours prior to the examination the diet should be low in fat. Dinner on the eve of the analysis should be light. It is recommended to avoid hypothermia and overheating, as well as physical and psychological stress.

Thyroid hormones 

• 4 weeks before the test, stop taking all medication that affects the thyroid function. When there is repeated analysis, stop taking the drugs on the day of the analysis. Be sure to tell your doctor and the technician about this, as well as any other medication that you are taking.
• ACTH, cortisol
• ACTH and cortisol are stress hormones and their level is subject to cyclical changes. The most informative results can be obtained as a result of research conducted before 9 am.A calm balanced emotional state is recommended with no stress, as the unmotivated release of hormones in the blood will cause an increase in level.

Sex hormones

• When this study is done, it is necessary to consider the stage of the menstrual cycle.Consult your doctor on which day of the menstrual cycle a particular hormone can be tested for. It is important to do the analysis in the morning in a calm emotional state. 

Tumor markers

General PSA, free PSA

• Blood for determination of PSA can be taken no earlier than 2 weeks after the biopsy of the prostate and prostate massage. Determination of post-surgical PSA is done not less than 6 weeks after surgery.

  Blood test for the presence of infections

Be sure to stick to a low-fat diet for 2 days prior to analysis.  
It is recommended to avoid eating citrus fruit or orange colored vegetables for 2 days prior to the analysis for viral hepatitis. The analysis for the presence of IgM antibodies should be performed within a week from the onset of the disease. The analysis for the presence of antibodies classes IgG, IgA after 10-14 days from the onset.

The basic rule of our work is high precision and high quality analysis.  

The equipment is serviced by a LIS (Laboratory Information System) program, assigning the patient a bar code during registration. This ensures confidentiality of information and the ability to send the processed results of analysis to their place of sampling in electronic form. Using the barcode LIS program also eliminates the possibility of errors due to human factors.  

All analyzers are installed in the Tibbiyot Dunyosi clinical diagnostic laboratory. Modern standards of diagnosis recommended by the World Health Organisation are adhered to. The equipment, reagents, calibrators and controls are registered with the Pharmacological Committee of the Ministry of Health of the Republic of Uzbekistan.

Daily internal quality control checks are carried out in the laboratory.

We perform external laboratory quality control with leading laboratories in Tashkent  such as Salus Vita Medical Center, Power Energy Central Laboratory and others.

All control measures are recorded.

If necessary, the Tibbiyot Dunyosi Brigade conducts sampling of biological material at home or in the office. Biomaterial delivery to the laboratory is carried out according to strict rules and standards. Samples are transported using special containers which ensures the stability of the biological material, and therefore also the quality, reliability and accuracy of the results. 

Working with samples in the PCR laboratory is carried out in a special box with special conditions and sterility. The DNA in the tube is prevented from entering the room in order to avoid false results for the other samples. All methods are highly sensitive and specific.

Shelf life of reagents is strictly controlled. Use of a reagent after its expiry date is forbidden. Test kits are used strictly for their intended purpose and in accordance with the instructions. Each test kit has its own serial number and mixing it with a different serial number is not allowed.

For each stage of research there are individual types of control. All data is stored in the computer and if necessary can be used in the preparation of test results that are questionable or if there are differences in the data obtained from other laboratories.  

Quality control covers the entire process of the analysis, from the biomaterial intake, its transportation and storage up to the finished results. Thus, quality control is carried out at all stages of laboratory and diagnostic procedures in order to avoid incorrect results.

In the near future we plan to obtain the ISO 9001 standard for clinical laboratory diagnostics. 

Implementation of external and internal quality control research gives us reason to ensure the accuracy of the research.

The laboratory is equipped with high-tech expert class medical equipment from leading manufacturers. Thanks to modern equipment and fully-automated processes, we can guarantee our patients the most accurate research results without errors.

Spectrophotometer Reflotron plus and COBAS C 111 from Roche Diagnostics GmbH (Germany) and Hitachi High Technologies (Japan) have a capacity up to 100 tests per hour with a wide range of studies designed for biochemical research.  

Automatic chemiluminescent analyzer Cobas e-411 is produced by Roche Diagnostics GmbH (Germany) and Hitachi High Technologies (Japan) for immunochemical studies. The quality and speed of execution and a wide range of research – more than 70 tests –  allows the test in 18 minutes. Designed to determine thyroid hormones, adrenal, reproductive system, pituitary, diagnosis of antiphospholipid syndrome, bone metabolism markers and osteoporosis tumor markers. 

Automatic analyzer Sysmex XS-1000 and Poch-100 i Sysmex made ​​in Japan are designed to study the general analysis of blood and provide a 24 parameter count of eosinophils, basophils, and a complete blood count. In addition there are 20 options for quantitative and qualitative evaluation of blood cells. Performance of both hematology analyzers is 120 tests per hour.

ESR Analyzer Human produced in Germany is used to diagnose the hemostatic system, inflammation, assessing the effectiveness of treatment and determination of ESR in 15 minutes. 

Semi-automatic Human analyzer is produced in Germany and is designed for rapid determination of fibrinogen, prothrombin, PTI, thrombin time and INR.  

Express urine analyzer Cobas-U 411 Roche is produced by Roche Diagnostics GmbH (Germany) and Hitachi High Technologies (Japan) and used to determine the 10 main indicators of urine and is a clinically proven reference technology for quick and easy identification of the indicators of urine.

Semi immuno-enzymatic ELISA analyzers Humareader HS, Combiwash from the Human company (Germany) is used in ELISA to diagnose HIV, TORCH infections, hepatic complex, giardiasis and ascariasis.  

Complex automated microscopy with the  Parasitology software module PARAS, is the procedure for a comprehensive survey on helminth infections and protozoa using automated microscopy and advanced technologies. 

The Thermocycler 6 plex QIAGENE Q ( fromGermany) is used to carry out a set of PCR (polymerase chain reaction technology) for Real Time diagnosis of viral hepatitis (B, C, D), the diagnosis of STIs: chlamydia, ureaplasma, mycoplasma, herpes virus infection, human papillomavirus which has a high carcinogenic risk, and others. 

The semi-automatic coagulometer HumaClot Junior Human firms (from Germany) is used to study the hemostatic system in the diagnosis of various pathological conditions.

The feasibility of the project

Tibbiyot Dunyosi Laboratory,  with equipment from leading manufacturers, ensures highly accurate analysis. The equipment is serviced by an * LIS program, assigning the patient a bar code when registering. Total confidentiality of information is assured and the system is able to send analysis results to the place of sampling electronically. Using the barcode LIS program also eliminates the possibility of errors due to human factors. 

Service at such a high level in government hospitals and clinics is not possible due to the lack of required laboratory and diagnostic equipment.

In addition, the sampling venue at a medical facility in the community will save a patient time because of the delivery of the analysis and findings to an external laboratory where his sample was taken. The patient will not have to go to the lab twice, and then back to the reception to his doctor.

At the centres of analysis we only offer those kinds of analyses that the clinics and hospitals where these centres are located can not carry out on their own. This will make it easier to monitor the competitiveness and the quality of the analyses. Our plan is to provide possible assistance to medical facilities in the territories where a venue for biomaterials collection exists.

• Free analysis in the amount of 2% of the volume of our services
• Free laboratory products for laboratories in the amount of 50 million soums
• An annual event for medical institutions using spatulas – a donation of one million spatulas in the amount of 234 million soums
• Free supply of hub cutters and needles and sharps containers to reduce the risk of infecting medical personnel.
• Free vaccination of the medical staff of health facilities exposed to the risk of contracting hepatitis B
• On the spot sampling tests and immediate results for patients critically ill in medical establishments.
• Organize seminars with the participation of well-known scientists to improve the skills of doctors in different areas of the health system

Part of the profits from the activity analysis at the centres of bio-material collection at medical and preventative treatment facilities of Tashkent and Tashkent region, we plan to use to finance the operation of two plants with five incinerators for the destruction of medical waste. The plants are of social importance because their realization will effectively help to fight the spread of infectious and viral diseases.

Centres for sampling tests at health care facilities in Tashkent

The Tibbiyot Dunyosi Medical Center Clinical diagnostic laboratory is equipped with central sterilization departments. Staff of the Centre only works with disposable consumables. We carry out a wide range of research with more than 300 tests and within six months we plan to increase this number to 500. The main range of studies (more than 200 tests) is carried out within one working day, while some emergenct tests can be performed in 1 – 3 hours.

We offer our patients:

• Biochemical studies of blood and urine for the following:

Bio-refineries (17 parameters) 
Ferments and enzymes 
Glucose carbohydrate metabolism and predisposition to diabetes
Creatinine, urea and uric acid 
Cholesterol, triglycerides and assessment of lipid profile
Total bilirubin, direct 
Micro and macro elements

• Immunochemical studiesinclude determining a blood marker of the thyroid gland, the pituitary-adrenal system, reproductive system assessment and monitoring of pregnancy, chromosomal screening for fetal abnormalities, anemia, osteoporosis, cardiac markers, hepatic profile evaluation for (hepatitis A, B, C, and antigens thereof and antibodies), detection of cancer markers in the blood that are specific to tumors of different organs:

HCG (human chorionic gonadotropin) – trophoblastic tumors of the uterus, testicular teratoma, ovarian horionepitelioma 
CA-15-3 – tumors of the breast, ovary, prostate, lung, stomach 
CA 125- ovarian tumors, uterine, endometrial 
PSA – prostate cancer 
NCE – lung tumors , neuroblastoma, leukemia 
CA 72-4 – stomach cancer 
Hormonal studies – laboratory evaluation of thyroid function 
Evaluation of pituitary-adrenal axis 
Hormonal assessment of the reproductive system and monitoring of pregnancy 
Screening for fetal chromosomal pathology 
Markers of anemia 
Immunologic studies 
Rank hepatic profile (hepatitis A, B, C, their antigens and antibodies)

• Proteins and cardiac risk markers for cardiovascular disease:

Troponin T – a specific analysis for the detection of focal lesions in acute myocardial ischemia, ACS (Acute coronary syndrome)
Myoglobin – cardiospecific analysis in acute myocardial ischemia, ACS (Acute coronary syndrome)
Enzymes CK and CK-MB – for differential diagnosis of acute myocardial infarction and cardialgia 
C-reactive protein – for the diagnosis of acute myocardial infarction and acute coronary syndrome

• Hematologic blood tests to count eosinophils, basophils, and a complete blood count. A quantitative and qualitative evaluation of blood cells to determine the ESR within 15 minutes to diagnose the hemostatic system, inflammation and assessing the effectiveness of therapy.
• Coagulology – rapid determination of fibrinogen, prothrombin, PTI, thrombin time, INR.
• Clinical tests- rapid diagnosis to determine the 10 main indicators of urine.
• IFA-diagnostics – the definition of HIV, TORCH infections, hepatic complex, giardiasis, ascariasis.
• PCR laboratory

Diagnosis of viral hepatitis B, C, D – quality, quantification, genotyping. 
Diagnosing STIs: chlamydia, ureaplasma, mycoplasma, herpes virus infection (HSV1, 2 types), HPV (human papillomavirus high carcinogenic risk) and others.

• Parasitology – research on parasites and protozoa:

Immunoassay for the detection of antigens in stool, Giardia Cryptosporidium (fecal antigens) 
Scatological diagnosis of pathogenic intestinal protozoa: Giardia, amoeba, cryptosporidium, blastocysts, intestinal helminth infections and other types of studies. 
Macroscopic and microscopic studies of parasitic pathogens, material content fragments and whole helminths 
Serological diagnosis of amebiasis, toxoplasmosis, toxocariasis, trihenelleza, ascariasis, giardiasis, cryptosporidiosis 
PCR diagnostics: Cryptosporidiosis, Leishmaniasis, simple, Toxoplasmosis

 A special place in the heart of Tibbiyot Dunyosi Medical Center is occupied by the clinical diagnostic laboratory. It is equipped according to European standards, in accordance with WHO recommendations. It is a unique research center of advanced technology laboratory medicine of which we are rightly proud. 

Here, are modern automatic analyzers of the latest generation from the world leaders in the field of Laboratory Instrumentation (ROCHE diagnostics, Sysmex, Hitachi, Human, Qiagen) This equipment gives expert results with an accuracy of 99.9% and avoids the errors associated with manual manipulation.

   The main principle of the laboratory is delivering a high quality of research and immediate delivery of the results. The equipment is serviced by an * LIS program, assigning the patient a bar code when registering. Total confidentiality of information is assured and the system is able to send analysis results to the place of sampling electronically. Using the barcode LIS program also eliminates the possibility of errors due to human factors. 

    The spectrum of laboratory tests carried out in the clinical diagnostic laboratory today is more than 300 tests, including biochemical, immunochemical, parasitological diligence, cardiac markers, ALLERGODIAGNOSTICS, drug monitoring, coagulogy and many others. In the next six months, the number of ongoing studies will increase to 500 tests. 

   In the Tibbiyot Dunyosi Medical Center there is a Real Time PCR-laboratory. Real-Time PCR is the latest technique to study the genomes of pathogens at the level of DNA and RNA and is widely used in the diagnosis of infectious diseases. It can detect even a single microorganism in the human body.  Real Time Technology is an analysis that gives instant results such as the pathogen quantitative content, the elimination of false-positive results, pre-clinical and retrospective diagnosis of infections in the incubation period and diagnosis of not only acute but latent infections as well. Real Time PCR is performed in the Tibbiyot Dunyosi laboratory by means of the advanced six channel thermal cycler Rotor-Gene Q, manufactured by QIACENE (Germany).

PCR is effective for the diagnosis of viral hepatitis B, C, D, bacterial and viral infections such as Helicobacter pylori and is used in gastroenterology, urology, gynecology, pulmonology, neurology, oncology, hematology, neonatology, forensic science, clinical genetics, and other areas of medicine .

Working with samples in the PCR laboratory is carried out in a special box with special conditions and sterility. The DNA in the tube is not exposed to the   room in order to avoid false results for the other samples. All methods are highly sensitive and specific and focused on prevention and early diagnosis of diseases in the incubation period.

Tibbiyot Dunyosi has established 5 blood sampling venues in Tashkent. These venues are located in State hospitals and clinics for the convenience of patients. High quality diagnosis and precision is guaranteed. Tibbiyot Dunyosi  plans to open an additional number of venues In the near future, bringing the total number of venues to 10 – 12. Currently, work is under way on the organization of the Tibbiyot Dunyosi Center branches throughout Uzbekistan. Branches will be opened  in Ferghana, Andijan, Bukhara, Khorezm and Termez.

Tibbiyot Dunyosi Clinical diagnostic laboratory offers the highest accuracy in results and a wide range of research, performed in the shortest time.

* Laboratory Information System

All analyzers and reagents are registered in the Republic of Uzbekistan; automatic analyzers installed in the clinical diagnostic laboratory meet all the modern standards of diagnosis recommended by the WHO.